Localization of Label-Retaining Cells in Murine Vocal Fold Epithelium PurposeEpithelial homeostasis is critical for vocal fold health, yet little is known about the cells that support epithelial self-renewal. As a known characteristic of stem cells is that they are slow-cycling in vivo, the purpose of this prospective controlled study was to identify and quantify slow-cycling cells or putative stem ... Article
Article  |   August 01, 2011
Localization of Label-Retaining Cells in Murine Vocal Fold Epithelium
 
Author Affiliations & Notes
  • Ciara Leydon
    Brooklyn College, New York, NY
    Brooklyn College, New York, NY
  • Rebecca S. Bartlett
    University of Wisconsin—Madison
    University of Wisconsin—Madison
  • Drew A. Roenneburg
    University of Wisconsin—Madison
    University of Wisconsin—Madison
  • Susan L. Thibeault
    University of Wisconsin—Madison
    University of Wisconsin—Madison
  • Ciara Leydon is now with the University of Wisconsin—Madison.
    Ciara Leydon is now with the University of Wisconsin—Madison.×
  • Correspondence to Susan L. Thibeault: thibeault@surgery.wisc.edu
  • Editor: Anne Smith
    Editor: Anne Smith×
  • Associate Editor: Roger Chan
    Associate Editor: Roger Chan×
Article Information
Speech, Voice & Prosody / Speech
Article   |   August 01, 2011
Localization of Label-Retaining Cells in Murine Vocal Fold Epithelium
Journal of Speech, Language, and Hearing Research, August 2011, Vol. 54, 1060-1066. doi:10.1044/1092-4388(2010/10-0267)
History: Received September 27, 2010 , Accepted December 22, 2010
 
Journal of Speech, Language, and Hearing Research, August 2011, Vol. 54, 1060-1066. doi:10.1044/1092-4388(2010/10-0267)
History: Received September 27, 2010; Accepted December 22, 2010
Web of Science® Times Cited: 7

PurposeEpithelial homeostasis is critical for vocal fold health, yet little is known about the cells that support epithelial self-renewal. As a known characteristic of stem cells is that they are slow-cycling in vivo, the purpose of this prospective controlled study was to identify and quantify slow-cycling cells or putative stem cells in murine vocal fold epithelium.

MethodTwelve mice were administered daily intraperitoneal injections of a nucleotide dye, bromodeoxyuridine (BrdU), over 7 consecutive days. Under this pulse-chase paradigm, slow-cycling cells retain the dye (label-retaining cells; LRCs) while more rapidly cycling cells lose dye to dilution during multiple cell divisions. The percentage of label-retaining cells (%LRCs) was calculated following a chase period of 2, 4, and 8 weeks postinjections.

ResultsThe %LRCs decreased significantly from 9.4% at 2 weeks to 3.1% at 8 weeks following injections (p < .05). No statistically significant differences in the quantity of BrdU-positive cells were measured between the anterior, mid-membranous, or cartilaginous regions of the vocal fold (p > .05).

ConclusionThese findings are consistent with the presence and first report of a small population of putative stem cells along the length of murine vocal fold epithelium.

Acknowledgments
This research project was supported by National Institute on Deafness and Other Communication Disorders Grant R01 DC004336. We extend our gratitude to Glen E. Leverson for assistance with statistical analysis and to Supriya D. Hayer and Michael Stitgen for assistance with data collection.
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